Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa

Abstract

This paper presents a comparison between batch and three different sets of fed batch fermentations for<br />rhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culture<br />medium having the initial glycerol and sodium nitrate concentrations of 30 and 8.3 g/l, respectively. For a fed<br />batch run with nitrogen source in feed, 250 ml of the nitrogen excluded culture medium was in the bioreactor<br />initially, and 250 ml culture medium containing 16.6 g/l sodium nitrate was fed to the bioreactor continuously.<br />A similar procedure was repeated for fed batch runs with carbon, and phosphorus source in feed. Statistical<br />analysis showed that fed batch runs were better than batch in term of rhamnolipid production, and among<br />the fed batch runs the maximum amount of rhamnolipid. (4.12 g Rhamnose Equivalent/l) was for the fed<br />batch run with the carbon source in feed.