Volume 10, Issue 4
مرور بر اساس
ارسال های اخیر
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Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)Efforts to express lipase in the periplasmic space of Escherichia coli have so far been unsuccessful andmost of the expressed recombinant lipases accumulate in the insoluble cell fraction. To evaluate the role of
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Expression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)Currently several studies are being carried out on various properties of mesenchymal stem cells (MSCs)however there are a few investigations about drug metabolizing properties of these cells. The aim of thisstudy ...
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Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration and
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Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)This paper presents a comparison between batch and three different sets of fed batch fermentations forrhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culturemedium ...
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Cloning, expression, purification and immunoreactivity analysis of gag derived protein p17 from HIV-1 CRF35 in fusion with thioredoxin from human subjects
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic ...
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Production and evaluation of polyclonal rabbit antihuman p53 antibody using bacterially expressed glutathione S-transferase-p53 fusion protein
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 ...
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Microbial cell surface display; its medical and environmental applications
(National Institute of Genetic Engineering and Biotechnology, 2012-10-01)Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them tofunctional components of cells which are exposed to the environment of cells. This strategy can be ...
