Correlation between E-Cadherin-Regulated Cell Adhesion and Human Osteosarcoma MG-63 Cell Anoikis

Abstract

Purpose: The aim of this study was to investigate the relationship between cell adhesion and anoikis evasionamong human osteosarcoma cells (MG-63), and to further study the molecular mechanisms. Materials and<br/><b>Methods</b>: Human osteosarcoma cells (MG-63) were assessed for apoptosis, and caspase-3, E-cadherin andβ-catenin expression in EDTA and control non-EDTA groups. <br/><b>Results</b>: MG-63 cells were predominantlyaggregated when in suspension, and the suspended cells were more dispersed in the EDTA group. Followingculture in suspension for 24 h, 48 h, or 72 h, the rates of apoptosis were 34.88%±3.64%, 59.3%±7.22% and78.5%±5.21% in the experimental group and 7.34%±2.13%, 14.7%±3.69%, and 21.4%±3.60% in the controlgroup, respectively. Caspase-3 expression progressively increased and E-cadherin and β-catenin were decreasedin the experimental group, whereas there was no change in the control group. <br/><b>Conclusions</b>: MG-63 cells couldavoid anoikis through cell adhesion, and E-cadherin might play a role in this process.