SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells

Abstract

<b>Background:</b> Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependentdeacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such asaging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function asa tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovariancarcinoma cells and its potential mechanism. Materials and <br/><b>Methods</b>: Expression of SIRT7 in ovarian cancercell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression wereconstructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate thebiological function of SIRT7 in ovarian cancer cells. <br/><b>Results</b>: SIRT7 demonstrated a higher level in ovariancancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reducedovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand,up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced changein apoptosis-related molecules and subunits of the NF-κB family. <br/><b>Conclusions</b>: In the present study, our dataindicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.