TP53 Codon 72 Polymorphisms and Lung Cancer Risk in the Bangladeshi Population

Abstract

<br/><b>Objective</b>: To assess associations between codon 72 polymorphisms (Pro or B and Arg or b alleles) of theTP53 gene and lung cancer risk among Bangladeshis. Materials and <br/><b>Methods</b>: The distribution of the BB, Bb,and bb genotypes and the frequencies of the B and b alleles were determined by PCR-RFLP method using DNAextracted from leucocytes of 50 confirmed lung cancer patients and 50 age-matched controls and the data wereanalysed. <br/><b>Results</b>: The ratio of BB, Bb, and bb genotypes were in Hardy-Weinberg equilibrium except for themale patients (χ2=4.6). The B allele is overrepresented among all patients (OR=2.0, p=0.02) and the femalepatients (OR=4.1, p≤0.01) compared to the controls. The BB/bb ratio was also higher among the patients (OR=3.0,p=0.03). The relative risk of cancer for having BB over bb genotype was 1.8 (p=0.04) but no effect was observedfor the Bb genotype. The B allele was overrepresented among patients with adenocarcinomas (OR=2.4, p≤0.01)and squamous cell carcinomas (OR=2.7, p≤0.01) over the controls but the difference was not significant for thosewith small cell lung carcinomas (OR=1.1, p=0.66). The B allele was overrepresented among patients age 50 oryounger (OR=2.7, p≤0.01), but not for older patients (OR=1.7, p=0.07), and among smokers compared to thecontrols (OR=1.8-10.0, p≤0.01-0.03). However, no correlation between increasing pack-years and lung cancerwas observed. <br/><b>Conclusions</b>: The Pro/Pro (BB) genotype and the B allele are risk factors for lung cancer amongBangladeshis, particularly for people under age 50, women and smokers.