• ورود به سامانه
      مشاهده مورد 
      •   صفحهٔ اصلی
      • نشریات انگلیسی
      • Asian Pacific Journal of Cancer Prevention
      • Volume 13, Issue 7
      • مشاهده مورد
      •   صفحهٔ اصلی
      • نشریات انگلیسی
      • Asian Pacific Journal of Cancer Prevention
      • Volume 13, Issue 7
      • مشاهده مورد
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Effects of Multiple-target Anti-microRNA Antisense Oligodeoxyribonucleotides on Proliferation and Migration of Gastric Cancer Cells

      (ندگان)پدیدآور
      پدیدآور نامشخص
      Thumbnail
      دریافت مدرک مشاهده
      FullText
      اندازه فایل: 
      715.8کیلوبایت
      نوع فايل (MIME): 
      PDF
      نوع مدرک
      Text
      زبان مدرک
      English
      نمایش کامل رکورد
      چکیده
      Backgrounds: To investigate the inhibiting effects of multi-target anti-microRNA antisense oligonucleotide(MTg-AMOs) on proliferation and migration of human gastric cancer cells. Methods: Single anti-microRNAantisense oligonucleotides (AMOs) and MTg-AMOs for miR-221, 21, and 106a were designed and transfectedinto SGC7901, a gastric cancer cell line, to target the activity of these miRNAs. Their expression was analyzedusing stem-loop RT-PCR and effects of MTg-AMOs on human gastric cancer cells were determined using thefollowing two assay methods: CCK8 for cell proliferation and transwells for migration. Results: In the CCK-8cell proliferation assay, 0.6 μmol/L was selected as the preferred concentration of MTg-AMOs and incubationtime was 72 hours. Under these experimental conditions, MTg-AMOs demonstrated better suppression of theexpression of miR-221, miR-106a, miR-21 in gastric cancer cells than that of single AMOs (P = 0.014, 0.024;0.038, respectively). Migration activity was also clearly decreased as compared to those in randomized and blankcontrol groups (28 ± 4 Vs 54 ± 3, P Conclusions: MTg-AMOs can specificallyinhibit the expression of multiple miRNAs, and effectively antagonize proliferation and migration of gastriccancer cells promoted by oncomirs.
      کلید واژگان
      MicroRNAs
      Stomach neoplasms
      oligonucleotides
      antisense
      Cell proliferation
      migration

      شماره نشریه
      7
      تاریخ نشر
      2012-07-01
      1391-04-11
      ناشر
      West Asia Organization for Cancer Prevention (WAOCP)

      شاپا
      1513-7368
      2476-762X
      URI
      http://journal.waocp.org/article_26647.html
      https://iranjournals.nlai.ir/handle/123456789/35901

      مرور

      همه جای سامانهپایگاه‌ها و مجموعه‌ها بر اساس تاریخ انتشارپدیدآورانعناوینموضوع‌‌هااین مجموعه بر اساس تاریخ انتشارپدیدآورانعناوینموضوع‌‌ها

      حساب من

      ورود به سامانهثبت نام

      تازه ترین ها

      تازه ترین مدارک
      © کليه حقوق اين سامانه برای سازمان اسناد و کتابخانه ملی ایران محفوظ است
      تماس با ما | ارسال بازخورد
      قدرت یافته توسطسیناوب