Biological Response Modifiers Influence Structure Function Relationship of Hematopoietic Stem and Stromal Cells in a Mouse Model of Leukemia

Abstract

Biological response modifiers (BRMs) can alter interactions between the immune system and cancer cells toboost, direct, or restore the body’s ability to fight disease. Mice with ethylnitrosourea- (ENU) induced leukemiawere here used to monitor the therapeutic efficacy of lipopolysaccaride (LPS), Bacillus Calmette Guerin (BCG)and sheep erythrocytes (SRBC). Flow cytometry based CD34+ positivity analysis, clonogenicity, proliferationand ultrastructure studies using scanning electron microscopy (SEM) of stem cells in ENU induced animals withand without BRMs treatment were performed. BRMs improved the stem-stromal relationship structurally andfunctionally and might have potential for use as an adjunct in human stem cell therapy.