Comparative Study of Correlation between Angiogenesis Markers (CD31) and Ki67 Marker with Behavior of Aggressive and Nonaggressive Central Giant Cell Granuloma with Immunohistochemistry Technique

Abstract

Background: The central giant cell granuloma (CGCG) is generally considered a non-neoplastic lesion. However,<br />some cases show aggressive behavior like neoplasms. Based on clinical observations, a number of researchers have<br />classified this lesion into aggressive and non- aggressive types. This study was aimed to investigate the association<br />between clinical behavior and histopathological features using immunohistochemical vascular CD31 and cellular<br />proliferation Ki67 markers. Materials and methods: In this descriptive-analytical, clinicopathological and<br />immunohistochemical study, 50 CGCGs, including 25 aggressive and 25 non-aggressive types were selected according<br />to Chuong's classification. The samples were then subjected to immunohistochemical staining to analyze positivity for<br />CD31 and Ki67 markers. Numbers of blood vessels and percentage proliferation of underlying fibroendothelial cells<br />were assessed, and the obtained results were analyzed with the t-test and the Mann-Whitney test. Results: The results<br />showed a significant difference between aggressive and non-aggressive CGCG lesions in the mean incidences of Ki67<br />(p=0.044). and CD31 (p=0.003) positivity. Conclusion: The present evaluation of expression rates for the vascular<br />CD31 and cellular proliferation Ki67 markers showed there might be a positive relation between the clinical features<br />and histopathology of CGCG. Furthermore, clinical behavior may be predicted based on features such as the number<br />of blood vessels and proliferation of fibroendothelial cells.