Repairing effects of interleukin 11 (IL-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on T-lymphoblastic leukemia cell line
(ندگان)پدیدآور
Han, YueqinZhu, YanpingWang, JinshenHan, YanqinQin, DaogangYang, QiaozhiSun, XiaojingChen, Lijunنوع مدرک
TextOriginal Article
زبان مدرک
Englishچکیده
Objective(s): To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. Materials and Methods: 95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), the IL-11-pre-treated high-dose group (C), the post-IL-11-treatment high-dose group (D) and the post-IL-11-treatment low-dose group (E). After the intraperitoneal injection of MTX in the groups B-E, the rats were sacrificed at 1, 3, 5 and 7 days. The mortality, morphological and ultrastructural changes of small intestine of each group were observed. The cells were then cultured in vitro, and the MTT method was used to investigate the effects of different concentration of IL-11 on CEM proliferation and also on HDMTX-induced mucositis. Results: IL-11 could reduce the intestinal histopathological score, increase the height of small intestinal villi, promote the proliferation of intestinal lacunar cells and reduce the mortality rate of rats. The IL-11 pre-treatment group exhibited the best efficacies, demonstrating significant difference with the control group (PConclusion: IL-11 could reduce the severity of HDMTX-induced intestinal mucositis, and improve the survival rate of experimental rats, and could be safely used as the adjuvant treatment of HDMTX in childhood leukemia.
کلید واژگان
CEM cell lineInterleukin 11
Methotrexate
Mucositis
شماره نشریه
7تاریخ نشر
2016-07-011395-04-11
ناشر
Mashhad University of Medical Sciencesسازمان پدید آورنده
Department of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, ChinaDepartment of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China
Department of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China
Departments of Pharmacy, the Fourth People's Hospital of Liaocheng, Liaocheng 252000, Shandong, China
Department of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China
Department of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China
Department of Pediatrics, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China
Department of Pediatrics, Shandong Province-owned Hospital, Jinan 250021, Shandong, China
شاپا
2008-38662008-3874