MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Abstract

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examinedroles in inhibiting growth and migration of MCF-7 cells. <br/><b>Methods</b>: MiR-886-5p mimics and inhibitors were usedto express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine thesurvival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expressionof caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, andthe levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration wasdetermined by wound healing and Transwell assays. <br/><b>Results</b>: We found that the growth of MCF-7 cells wasinhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis anddecreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, andMMP9 was also found to be decreased as compared to controls. <br/><b>Conclusions</b>: Our data show that downregulationof miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might implythat inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.