Improved protocol for isolation of genomic DNA from leaf tissues of Phyllanthus emblica Gaertn

Abstract

Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissues<br />of Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturity<br />were analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.<br />tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pH<br />was the primary cause for poor quality of DNA. However, high quality DNA isolation was achieved by<br />stabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocol<br />yielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes for<br />molecular analysis.