Effect of Kinetin on Multiple Shoot Induction in Cotton (Gossypium hirsutum L.) cv. NIAB-999

Abstract

Multiple shoot induction was studied in upland cotton cv. NIAB-999. Cotyledonary nodes obtained from<br />aseptically raised seedling were cultured on modified Murashige and Skoog medium (MS) supplemented<br />with different doses of Kinetin. Cotyledonary nodes produced maximum number of shoots (3.43 shoots/<br />explant) when cultured on MS medium supplemented with 0.25 mgl-1 Kinetin. Highest percentage (93.3 %) of<br />root development and root length (5.85 cm) was obtained when shoots were cultured on MS medium<br />supplemented with 0.5 mgl-1 napthalene acetic acid (NAA) and 0.1 mgl-1 Kinetin.<br /><br />