Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis

Abstract

The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized by<br />reverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coat<br />protein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibody<br />sandwich enzyme-linked immunesorbent assay (DASELISA) in a population of 330 symptomatic grapevine<br />leaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined.<br />Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.<br /><br />