Volume 6, Issue 4
مرور بر اساس
ارسال های اخیر
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Sequence Analysis of M2 Gene of Avian Influenza Virus Strain (A/Chicken/Iran/101/98 (H9N2)) as an Oil Vaccine Seed
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)In this study, the full-length M2 gene of the avian influenza virus (H9N2) was isolated, analyzed and studied in detail. Total RNA was extracted and cDNA of the M2 mRNA was obtained by reverse transcriptase polymerase chain ...
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Optimization of Transient Expression of uidA Gene in Androgenic Embryos of Wheat (Triticum aestivum L. cv. Falat) via Particle Pombardment
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the ...
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Phenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance ...
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Evaluation of Nucleic Acid Sequence Based Amplification (NASBA) and Reverse Transcription Polymerase Chain Reaction for Detection of Coxsackievirus B3 in Cell Culture and Animal Tissue Samples
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)Enteroviruses are the causative agents of a number of diseases in humans. Group B coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. Genomic data of enteroviruses has ...
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Site-Directed Mutagenesis in Human Granulocyte-colony Stimulating Factor, Cloning and Expression in Escherichia coli
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)Human granulocyte colony stimulating factor (hG-CSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have ...
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Isolation and Characterization of Thermophilic Alkaline Proteases Resistant to Sodium Dodecyl Sulfate and Ethylene Diamine Tetraacetic Acid from Bacillus sp. GUS1
(National Institute of Genetic Engineering and Biotechnology, 2008-10-01)Thermophilic Bacillus sp. GUS1, isolated from a soil sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram ...
