Exploring the Protective Effect of Ascorbic Acid and Aqueous Extract of Spirulina platensis on Methotrexate-Induced Lipid Peroxidation

Abstract

This study was designed to explore the protective effects of ascorbic acid and water extract of <em>Spirulina platensis</em> (blue green algae) on methotrexate-induced lipid peroxidation using goat liver as the lipid source. This <em>in vitro</em> evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of tissue homogenates. The study reveals the lipid peroxidation induction capacity of methotrexate, the anti-peroxidative potential of ascorbic acid and aqueous extract of <em>S. platensis</em> on methotrexate-induced lipid peroxidation.