Study of Direct Adventitious Shoot Regeneration in Pomegranate ( Punicagranatum cv. MalasSaveh ) through Cotyledonary Explants

Abstract

Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In this<br />study some experiments were conducted to introduce the best hormonal treatments for direct<br />adventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For this<br />purpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BA<br />at 2, 4.5, 9, 13 μM alone and in combination with 5.5 μM NAA or 5 μM 2,4-D were used. The<br />highest number of shoot per explant 8.2 and 7.9 were obtained in MS liquid and agar medium<br />supplemented with 13μM BA and 5.5 μM NAArespectively, although there was no significant<br />differences between two medium. The highest shoot length 1.65 cm was obtained from MS liquid<br />medium supplemented with13μM BA and 5.5 μM NAA as well. The highest rooting was obtained<br />in half-strength MS containing 2 mg/l IBA with 3.1 roots per explant. Rooting plantlets were<br />transferred to Jiffy and acclimatized after 35 days successfully.