Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells
(ندگان)پدیدآور
پدیدآور نامشخصنوع مدرک
Textزبان مدرک
Englishچکیده
The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethylacetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 humanlung adenocarcinoma epithelial cells. A-549 cells were exposed to 10-1000 μg/ml concentrations of the leaf extractsof A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyltetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced theviability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate,36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% withwater soluble extracts at 1000 μg/ml each. Among the various plant extracts, ethyl acetate extract showed thehighest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol,and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of differentsoluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of apotential therapeutic anticancer agents.
کلید واژگان
Abelia trifloraA-549 cell line
Cytotoxicity
MTT assay
شماره نشریه
11تاریخ نشر
2015-11-011394-08-10
ناشر
West Asia Organization for Cancer Prevention (WAOCP)شاپا
1513-73682476-762X