Expression Analysis of Serum microRNA-34a and microRNA-183 in Hepatocellular Carcinoma

Abstract

Background/objective: HCC is a multistep process starting from chronic hepatitis that progress through cirrhosis<br />to HCC. MicroRNA expression level was found to be deregulated in HCC. To find out whether the expression level of<br />miR-34a and miR-183 was deregulated in HCC compared to controls without HCC. Methods: Real time quantitative<br />PCR was done to find out the miRNA expression level in terms of Ct value followed by statistical analysis. Results:<br />Over-expression of miR-183 and under-expression of miR-34a in HCC was detected. All changes in expression level<br />of miR-34a and miR-183 were found to be due to HCC compared to controls without HCC. So both miR-34a and<br />miR-183 were suitable to differentiate HCC from Cirrhosis and chronic hepatitis with an efficient diagnostic power of<br />sensitivity, specificity and expression level. But they might not have any role in patients' survival. Conclusion: miR-<br />34a and miR-183 might be considered as potential markers of HCC screening molecule in addition to other approved<br />panel of marker. Our study warrants further expression level study.