PBK/TOPK Expression During TPA-Induced HL-60 Leukemic Cell Differentiation

Abstract

<br/><b>Objective</b>: This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cellsinduced by tetradecanoyl phorbol acetate (TPA). <br/><b>Methods</b>: Wright-Giemsa staining was performed to observemorphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b,CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis. <br/><b>Results</b>: Aftertreating HL60 cells with 5.1 × 10-9 mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positivecells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased. <br/><b>Conclusions</b>:TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage.PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression wasincreased.