G1/S-specific Cyclin-D1 Might be a Prognostic Biomarker for Patients with Laryngeal Squamous Cell Carcinoma

Abstract

<br/><b>Objective</b>: To investigate the prognostic role of antigen KI-67 (Ki-67) and G1/S-specific cyclin-D1 (cyclin-D1)in patients with laryngeal squamous cell carcinoma (LSCC). <br/><b>Methods</b>: Immunohistochemical staining (IHS)was used to determine the protein expression of Ki-67 and cyclin-D1 in LSCC tissues. Kaplan-Meier survivalcurves was calculated with reference to Ki-67 and cyclin-D1 levels. <br/><b>Results</b>: Cyclin-D1 and Ki67 were expressedin the nuclei of cancer cells. Among the total of 92 cancer tissues examined by immunohistochemistry, 60(65.22%) had cyclin-D1 overexpression and 56 (60.87%) had Ki67 overexpression. Cyclin-D1 overexpression isassociated with the advanced stage of the cancer (P=0.029), but not with gender, age, stage of cancer, histologicaldifferentiation, anatomical site, smoking history and alcohol consumption history. Ki67 overexpression is notassociated with the advanced stage, gender, age, histological differentiation, anatomical site, smoking history andalcohol consumption history. A statistically significant correlation was found between lymph node status and theexpression of Ki67 (p = 0.025). Overexpression of cyclin-D1 was correlated to shorter relapse-free survival period(P<0.001). <br/><b>Conclusions</b>: Overexpression of cyclin-D1 can be used as a marker to predict relapse in patients withLSCC after primary curative resection.