نمایش مختصر رکورد

dc.contributor.authorMard, Seyyed Alien_US
dc.contributor.authorVeisi, Alien_US
dc.contributor.authorAhangarpour, Akramen_US
dc.contributor.authorGharib-Naseri, Mohammad Kazemen_US
dc.date.accessioned1399-07-09T08:21:20Zfa_IR
dc.date.accessioned2020-09-30T08:21:20Z
dc.date.available1399-07-09T08:21:20Zfa_IR
dc.date.available2020-09-30T08:21:20Z
dc.date.issued2016-02-01en_US
dc.date.issued1394-11-12fa_IR
dc.date.submitted2016-02-28en_US
dc.date.submitted1394-12-09fa_IR
dc.identifier.citationMard, Seyyed Ali, Veisi, Ali, Ahangarpour, Akram, Gharib-Naseri, Mohammad Kazem. (2016). Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa. Iranian Journal of Basic Medical Sciences, 19(2), 172-177. doi: 10.22038/ijbms.2016.6541en_US
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.urihttps://dx.doi.org/10.22038/ijbms.2016.6541
dc.identifier.urihttp://ijbms.mums.ac.ir/article_6541.html
dc.identifier.urihttps://iranjournals.nlai.ir/handle/123456789/339857
dc.description.abstract<strong><em>Objective(s):</em></strong> This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H<sub>2</sub>S in gastric mucosa in rats. <strong><em>Materials and Methods:</em></strong>Thirty-two rats were randomly assigned into 4 groups (8 in each), including: the control group, HCl (10 mM) treated group, HCl (100 mM) treated group, and one group to study the effect of Nω-Nitro-L-arginine methyl ester hydrochloride<strong> </strong>(L-NAME). Anesthetized rats underwent tracheostomy and midline laparotomy. Ninety min after the instillation of neutral or acidic solutions, animals were sacrificed and the gastric mucosa was collected to measure the H<sub>2</sub>S concentration by ELISA method and to quantify mRNA expression of CSE and CBS by quantitative real-time PCR (qRT-PCR). Protein synthesis was also detected by Western blot method. <strong><em>Results:</em></strong>Mucosal acidification with 10 and 100 HCl, significantly increased mucosal levels of H<sub>2</sub>S (<em>P</em><0.01 and <em>P</em><0.001) and mRNA (<em>P</em><0.01 and <em>P</em><0.001) and protein expressions of CSE (<em>P</em><0.01 and <em>P</em><0.001). L-NAME treatment reversed H<sub>2</sub>S release to control level. <strong><em>Conclusion:</em></strong>Our findings indicated that mucosal acidification with HCl increased mucosal release of H<sub>2</sub>S through upregulation of CSE gene and its protein expression. This effect is mainly mediated through the involvement of nitric oxide.en_US
dc.format.extent1059
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_US
dc.publisherMashhad University of Medical Sciencesen_US
dc.relation.ispartofIranian Journal of Basic Medical Sciencesen_US
dc.relation.isversionofhttps://dx.doi.org/10.22038/ijbms.2016.6541
dc.subjectCystathionine gamma lyase H2Sen_US
dc.subjectMucosal acidificationen_US
dc.subjectRaten_US
dc.titleMucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosaen_US
dc.typeTexten_US
dc.typeOriginal Articleen_US
dc.contributor.departmentPhysiology Research Center, Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranen_US
dc.contributor.departmentPhysiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranen_US
dc.contributor.departmentPhysiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranen_US
dc.contributor.departmentPhysiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranen_US
dc.citation.volume19
dc.citation.issue2
dc.citation.spage172
dc.citation.epage177
nlai.contributor.orcid0000-0002-4323-1672
nlai.contributor.orcid0000-0003-3430-0354


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