Attenuation of N-nitrosodimethylamine induced hepatotoxicity by Operculina turpethum in Swiss Albino mice

Abstract

<br/> <br/> <br/> <br/> <br/><em><span style="font-size: xx-small;">Objective(s): </span></em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">To appraise the antihepatotoxic efficacy of ethanolic extract of </span></span><em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><em><span style="font-family: Cambria,Cambria; font-size: xx-small;">Operculum turpethum </span></em></span></em> <br/><span style="font-family: Cambria,Cambria; font-size: xx-small;">root on the liver of Swiss albino mice. </span> <br/> <br/>Materials and Methods: <br/> <br/><span style="font-family: Cambria,Cambria; font-size: xx-small;">Hepatic fibrosis was induced in adult male albino mice through intraperitoneal administrations of N-nitrosodimethylamine (NDMA) at the concentration of 10 mg/kg body weight. The liver toxicity and therapeutic effect of the plant ethanolic extract was assessed by the analysis of liver marker enzymes and antioxidant enzymes and liver histopathological studies. </span> <br/> <br/>Results: <br/><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">Hepatotoxicity was manifested by significantly decreased (</span></span><em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><em><span style="font-family: Cambria,Cambria; font-size: xx-small;">P</span></em></span></em> <br/><span style="font-family: Cambria,Cambria; font-size: xx-small;"><0.01) levels of the activities of the enzymatic and non enzymatic antioxidants such as superoxide dismutase, catalase, GSH and increased levels of cholesterol, AST, ALT, ALP and lipid peroxidation. The plant extract significantly restored the antioxidant enzyme level in the liver and exhibited significant dose dependent curative effect against NDMA induced toxicity which was also supported by histopathological studies of the liver. </span> <br/> <br/>Conclusion: <br/> <br/><span style="font-family: Cambria,Cambria; font-size: xx-small;"><em><span style="font-family: Cambria,Cambria; font-size: xx-small;">O. turpethum </span></em></span><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">manifested therapeutic effects by significantly restoring the enzymatic levels and reducing the hepatic damage in mice. This work intends to aid researchers in the study of natural products which could be useful in the treatment of liver diseases including cancer. </span></span>