microRNA Expression Profile in Patients with Stage II Colorectal Cancer: A Turkish Referral Center Study

Abstract

<b>Background:</b> There are increasing data about microRNAs (miRNA) in the literature, providing abundantevidence that they play important roles in pathogenesis and development of colorectal cancer. In this study, weaimed to investigate the miRNA expression profiles in surgically resected specimens of patients with recurrentand non-recurrent colorectal cancer. Materials and <br/><b>Methods</b>: The study population included 40 patients withstage II colorectal cancer (20 patients with recurrent tumors, and 20 sex and age matched patients withoutrecurrence), who underwent curative colectomy between 2004 and 2011 without adjuvant therapy. Expression of16 miRNAs (miRNA-9, 21, 30d, 31, 106a, 127, 133a, 133b, 135b, 143, 145, 155, 182, 200a, 200c, 362) was verifiedby quantitative real-time polymerase chain reaction (qRT-PCR) in all resected colon cancer tissue samples andin corresponding normal colonic tissues. Data analyses were carried out using SPSS 15 software. Values werestatistically significantly changed in 40 cancer tissues when compared to the corresponding 40 normal colonictissues (p<0.001). MiR-30d, miR-133a, miR-143, miR-145 and miR-362 expression was statistically significantlydownregulated in 40 resected colorectal cancer tissue samples (p<0.001). When we compared subgroups,miRNA expression profiles of 20 recurrent cancer tissues were similar to all 40 cancer tissues. However in 20non-recurrent cancer tissues, miR-133a expression was not significantly downregulated, moreover miR-133bexpression was significantly upregulated (p<0.05). <br/><b>Conclusions</b>: Our study revealed dysregulation of expressionof ten miRNAs in Turkish colon cancer patients. These miRNAs may be used as potential biomarkers for earlydetection, screening and surveillance of colorectal cancer, with functional effects on tumor cell behavior.