Expression and Significance of Microsomal Prostaglandin Synthase-1 (mPGES-1) and Beclin-1 in the Development of Prostate Cancer

Abstract

The aim of this study was to investigate the expression and significance of microsomal prostaglandin synthase-1(mPGES-1) and Beclin-1 in the development of prostate cancer (PCa). Immunohistochemistry was performed onparaffin-embedded sections with rabbit polyclonal against mPGES-1 and Beclin-1 in 40 PCa, 40 benign prostatichyperplasia (BPH) and 10 normal prostate specimens for this purpose. Quantitative real-time polymerase chainreaction (qRT-PCR) was applied for mRNA expression of mPGES-1 and Beclin-1, while MTT assays were usedto ascertain the best working concentration of the mPGES-1 inhibitor (CAY10526). The effect of CAY10526treatment on expression of Beclin-1 in DU-145 cells was studied using Western blot analysis. Localization ofBeclin-1 and mPGES-1 was in endochylema. Significant differences in expression was noted among PCa, BPHand normal issues (P<0.05). Beclin-1 expression inversely correlated with mPGES-1 expression in PCa tissue(P<0.05). CAY10526 could significantly block mPGES-1 expression and the proliferation of DU-145 cells (P<0.05),while increasing Beclin-1 levels (P<0.05). Overexpression of mPGES-1 could decrease the autophagic PCa celldeath. Inhibiting the expression of mPGES-1 may lead to DU-145 cell death and up-regulation of Beclin-1. Theresults suggest that inhibition of mPGES-1 may have therapeutic potential for PCa in the future.