siRNA Interference with a Proliferation-Inducing Ligand Gene in the Sgr-7901 Gastric Carcinoma Cell Line

Abstract

<br/><b>Objective</b>: The present study aimed to investigate the influence of siRNA interference with a proliferationinducingligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencingand tyrosine kinase (trka) expression were also explored. <br/><b>Methods</b>: Two APRIL-silencing siRNA vectors wereconstructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfectionbeing detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RTPCRand apoptotic rates of sgr-7901 were assessed by flow cytometry. <br/><b>Results</b>: The expression levels of receptortrka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased aftertransfection (P < 0.05). <br/><b>Conclusion</b>: APRIL gene silencing can increase the apoptotic rate of gastric carcinomacells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathwaysof APRIL and trka.