ТР53 Codon 72 Polymorphism and Human Papilloma Virus-Associated Cervical Cancer in Kyrgyz Women

Abstract

Background: The aim of this study was to ascertain the magnitude of association of gene ТР53 Arg72Pro polymorphic<br />marker with cervical cancer (CC) in Kyrgyz women. Methods: We identified and included 205 women of Kyrgyz<br />ethnicity for this case-control study, of whom N=103 were women (mean age 53.5 ± 10.0 years) with histologically<br />confirmed CC and N=102 controls (mean age 46.5 ± 8.5 years). We detected human papilloma virus (HPV) DNA types<br />16 and 18 using polymerase chain reaction (PCR) with hybridization/fluorescent detection. Genotypes of ТР53 gene<br />Arg72Pro polymorphism were identified using PCR-RFLP assay. Results: Eighty-eight percent (90/103) women with<br />CC had HPV, of whom 43.4% (39/90) had HPV type 16, 24.4% (22/90) had HPV type 18, whereas 32.2% (29/90)<br />carried both types. The univariate analysis of allele and genotype distribution of Arg72Pro polymorphic marker of ТР53<br />gene showed no difference between CC and control groups (χ2=1.24, р=0.54). However, when CC cases associated with<br />HPV were tested against controls, Arg72 allele and Arg72Arg genotype prevalence were greater compared to controls<br />(χ²=7.25; р=0.027 for genotypes and χ²=6.83; р=0.009 for alleles). In HPV-positive women, Arg72Arg genotype of<br />ТР53 gene was associated with a 1.85-fold increase in the likelihood of CC (OR=1.85 [95% confidence interval (CI)<br />1.03-3.32]), whereas Arg72 allele increased this likelihood 1.94-fold (OR=1.94 [95% CI 1.20-3.15]). Conclusions:<br />Arg72Arg genotype and Arg72 allele of ТР53 gene in Kyrgyz women increase the risk of HPV-associated CC.