AntagomiR-27a Targets FOXO3a in Glioblastoma andSuppresses U87 Cell Growth in Vitro and in Vivo

Abstract

<br/><b>Objective</b>: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. <br/><b>Methods</b>: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised duringdecompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27awas screened out through bioinformatics databases, and the predicted gene was verified using genetic reportassays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTTassays and 5-ethynyl-2’-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude micewas established to detect the effect of antagomiR-27a on tumour growth. <br/><b>Results</b>: qRT-PCR results showed thatmiR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. ThmiR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verifiedas a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressedby administration of the miR-27a inhibitor. <br/><b>Conclusion</b>: MiR-27a may be up-regulated in human glioblastoma,and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.