Comparison of the Expression Level of P53 and Proliferating Cell Nuclear Antigen (PCNA) Genes in the Inflamed and Non-Inflamed Dentigerous Cysts

Abstract

Background & Aims: The role of P53 and proliferating cell nuclear antigen (PCNA) genes in inflamed and non-inflamed odontogenic cysts is related to cell proliferation but it is unclear. The aim of this study was immunohistochemical evaluation of P53 and PCNA expression in inflamed and non-inflamed dentigerous cysts Methods: In the present study, 23 dentigerous cysts (12 inflamed and 11 non-inflamed) were surveyed. Three microns slices were got from paraffined blocks and stained with P53 and PCNA antibodies by immunohistochemistry. Slides were seen by oral pathologist with optical microscope (Olympus BX41) in ×400 magnification and scored by Allred scoring. Data were analyzed by Mann-Whitney test via SPSS software. Results: Percent of stained cells in inflamed compared non-inflamed dentigerous cyst did not show any significant difference for expression level of P53 and PCNA in basal and suprabasal layers (P> 0.05). Conclusion: It seems that inflammation does not affect on expression level of P53 and PCNA in dentigerous cyst.