Investigation of Consecutive Separating Arrangements of Bio active Compounds from Black Tea (Camellia sinensis) Residue

Abstract

Every year lots of black tea (<em>Camellia sinensis</em> (L.) Kuntze) residue will produce in the factories. These residue are unusable whereas the bio active compounds can be extracted and used in the drag and food industries. Due to mentioned problems, this project was conducted years 2011 - 2012 with the aim to make a study on consecutive isolation of all bio active compounds from tea residue, that extraction of one compound won't benefite to build a lateral products factory but isolation of all bio active compounds can increase productivity. In this survey, four compounds of caffeine, catechin, fiber and protein were be separated and measured from residue mixture in three steps from three sequential models. The isolation of caffeine and catechin were placed together in one step because the extraction condition was similar. Experiments was conducted with four replications and data was analyzed. The results indicated that effect of three sequence models was significant on extraction yield of caffeine, catechin, protein, and fiber (P<0/01). Comparison of yields indicated that the maximum amount of caffeine and protein was obtained from second sequence, also the maximum amount of catechin and fiber from third sequence. The economic comparison results among sequences indicated that the all sequences were economical however third sequence was introduced as the most economical model in terms ratio of benefit to cost due to high price of catechin and maximum rate.