Seroprevalence of leptospiral infection in feline population in urban and dairy cattle herds in Mashhad, Iran

Abstract

The importance of cats in the <em>Leptospira</em> epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. The purpose of this survey was to determine the prevalence of <em>Leptospira </em>infection in shorthair cats in different location of Mashhad, Iran. Totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households, stray and cats which lived in industrial dairy cattle herds of Mashhad, Iran, respectively. Sera were tested with seven live <em>Leptospira</em> antigens using microscopic agglutination test (MAT). Sera with 50.00% agglutination at the dilution of ≥ 1/100 were considered as positive samples. Agglutination at dilutions of < 1/100 considered as suspected to <em>Leptospira</em> infection. Overall, 19 (12.92%) out of 147 cats showed reaction in MAT. The seroprevalence at a titer ≥ 1:100 and < 1:100 were 10 (6.80%) and 9 (6.12%), respectively. Serum samples showed positive reaction against <em>Leptospira</em> <em>intterogans hardjo</em> (no = 10; 52.63%), <em>pomona</em> (no = 5; 26.31%) and <em>icterohaemorrhagiae </em>(no = 4; 21.05%). Eight cats (42.10%) belong to dairy cattle herds had the most infection only by <em>L. I. hardjo</em> with 1:200 titer. There were no significant differences among the weight‚ age and sex of infected cats. However, there were significant differences between the infected cats in dairy cattle herds and the cats in the urban area (<em>p</em> < 0.05). It is concluded that cats can be infected by <em>Leptospira</em> spp. especially in commercial dairy cattle herds. Cats can be considered as a sanitation hazards in the area for this zoonotic disease.