Utilizing a Three-phase Hollow Fiber Liquid-phase Micro Extraction Method for Metoprolol Succinate Extraction from Biological Urine Prior to HPLC-UV Analysis

Abstract

This study presents hollow fibers in liquid-phase micro extraction (LPME) method as a technique, in order to pre-concentrate Metoprolol Succinate drugs from tap water and urine samples. The extracted analyte with hallow fiber was detected with high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The effect of several parameters on extraction efficiency such as organic solvent type, receiving phase, pH of receiving and extractant phase, the extraction time and mixing speed were investigated and optimized. After extracting the Metoprolol Succinate drug under the optimum conditions such as types of extraction solvents, stirring speed, extraction time and solvent pH, the pre-concentration factor was obtained 192 for tap water and 169 for urine samples. The obtained detection limit was 3.90 μg/L for urine and 1.5 μg/L for tap water in which R2 > 0.995. The extraction recovery for real samples was 94% for urine sample and 98 % for tap water.