Design and Fabrication of PVC Membrane Electrode Based on Netural Ligand (E)-N'-(1-(2-Hydroxyphenyl)Ethylidene)Benzohydrazi for Teramadol hydrochloride Measurement in Drugs and Biological Fluids

Abstract

Here, a simple, quick, Low cost and sensitive Potentiometric method is described for tramadol hydrochloride measurement using .PVC membrane electrode based on neutral ligand (E)-N'-(1-(2-hydroxyphenyl)ethylidene)benzohydrazid. Besides, effect of various parameters like ionic additives, membrane solvent and pH on the Potentiometric response of the electrode are investigated. The best electrode performance was observed with membrane composition of PVC 30%, DBP60%, carrier 7% and additive3% within pH range of 3-6. This electrode showed Nernstian slope about -60.24±0.2 mV/dec, detection limit of 1.75×10-6 M and response time about 15 sec over the concentration range of 1.0×10-1 to 3.16×10-6 M. The lifetime of electrode was about 40 days. Finally, by using the electode in measuring the amount of tramadol hydrochloridein urine, cow's milk, and tablet utilizing calibration curve and method of standard addition, the electrode performance and behavior are investigated. The results showed the mentioned electrode had more appropriate performance than other usual approaches.