Searching for Alpha-Glucosidase Inhibitory Activity in Hexane Extracts by some Plants from Kurdistan Province

Abstract

Objective: Alpha-glucosidase, as a carbohydratase catalyses the liberation of alpha-glucose from the nonreducing end of some carbohydrates from foods in digestive tract. Consequently, retard absorption of glucose by the inhibition of carbohydrate hydrolyzing enzymes is one of the therapeutic approaches to decrease the postprandial hyperglycemia. Recently, a great deal of attention has been paid to natural products like plants, as a reliable source for bioactive compounds.The aim of this study was to find new potent inhibitors for Alpha-glucosidase among plant extracts. Methods: Hexan extracts of 60 plants species were screened for their Alpha-glucosidase inhibitory activity at 0.001, 0.01, 0.1 and 1 mg/mL concentration. Acarbose, dissolved in buffer, was used as a positive control. Every experiment was done in triplicate.Descurania Sophia (L.) Webb & Berth, Fumaria vailantii Loisel, Ferula Haussknechti Wolf ex Rech,Haplophyllum acutifolium (DC.)G.Don, Isatis cappadociaca Desv., Eremostachys laevigata Bunge, Silene aucheriana Boiss exhibited more than 60% alpha-glucosidase inhibitory activity.. Results: Among them, <em>Descurania Sophia</em>, <em>Fumaria vailantii </em>and <em>Ferula haussknechti</em> exhibited significant alpha glucosidase inhibitory activity with IC50 values of 0.9 µg/ml, 24.55 µg/ml and 2.71 µg/ml, respectively. Because of its high inhibitory activity and low IC50, <em>Descurania Sophia</em> extract would be interesting for further studies