Genetic Diversity of Phytophthora Sojae in Iran

Abstract

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of <em>P. sojae</em> from Iran. Methods: During 2005–2007, 142 isolates of<em> Phytophthora sojae</em> were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of <em>P. sojae</em> fifty isolates was amplified with 20 RAPD and 5 ISSR primers. Results: Five primers amplified 77 fragments, of which 20 were polymorphic, indicating low variability. RAPD and ISSR primers clustered isolates into three distinct groups at 85% similarity. Genetic diversity among isolates detected intra specific variability even among isolates of the same geographic origin. There is no correlation between races, geographical distribution, and molecular markers.