The Production Pattern of Aflatoxin G1, G2 , by HPLC

Abstract

Objective: Aflatoxin G₁and G_2, are highly toxic and carcinogenic secondary metabolites produced by <em>Aspergillus</em> <em>flavus</em> and <em>Aspergillus parasiticus</em> fungi sampling was done according to "CBS" instructions from indoor and outdoor stations. Methods: In HPLC measuring, we first purified our sample of all Aflatoxins, We used imonoaffinty columns, Finally the device compared average of the results of the samples curve areas with average of the results of standard curve areas and showed the effective material value based on ppb. Geographical distribution of 107 mentioned samples and The frequency of subgenuses in different regions, The minimum, average and maximum amount of toxins, The average amount of produced toxin in culture medium were determined. Results: In comparison, the average of aflatoxin G₁ and G₂with HPLC method in biomass the highest examined amount is in the range of -0/5 -0/5 ppb which is produced by fungi such <em>as A. niger, A. flavus, A. fumigatus</em>. In biomass, the correlation between aflatoxin G₁ and G₂ measured by HPLC method isn't any reverse relation and meaningful numeral differences. In culture medium, checking the average value of aflatoxin G₁ and G₂which is measured by HPLC method, we can see a correlation and there is a meaningful numeral difference.