Evaluation of The Effect of Antimicrobial Activity of Ethanol Extract of Calotropis Procera in Extended Spectrum Beta- Lactamase Producing E. Coli

Abstract

Infectious diseases are responsible for thousands of worldwide early death .Many commercial antibiotics have been used to control human's infectious diseases all over the world. <em>Calotropis procera</em> Linn a wild growing plant of Asclepiadaecae family is known to possess multifarious medicinal properties. Total 80 non repetitive clinical <em>E. coli </em>strains recovered during 7 month, and were screened for ESBL production by disc diffusion test, and the MIC and MBC of <em>Calotropis procera</em> chlorophyll extract against ESBL positive <em>E. coli </em>isolates were determined. The results showed a total of 30 of 80 (37.5%) isolates harboured ESBL enzymes.<em> Calotropis procera</em> <em>Calotropis procera</em> extract were effective against ESBL producing <em>E. coli </em>isolates. There is need for a correct and reliable phenotypic test to identify ESBL beta lactamases and also these bioactive plants may help alleviate the problem of drug resistance. The presence of these chemical constituents in this plant is an indication that the plant, if properly screened using additional solvents, could yield drugs with pharmaceutical significance