Optimization of Ultrasonic-Assisted Extraction of Natural Antioxidants from Elaegnus Angustifolia Using Response Surface Methodlogy

Abstract

Restrictions on the use of synthetic antioxidant such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are being imposed because of their carcinogecity. In this investigation optimization of phenolic extraction process frome methanolic (80%) extract (v/v) of elaegnus angustifolia through Response Surface Methodology by fulin cio- calteu is performrd for optimizing the optimizing the process three factor (time, temperature and solution to solid ratio) were investigated each factor was repeated three times (5,20,35 min), temperature (30, 45,60), and solution to solid ratio (20,35,50). The experiment was conducted based on Box-Behnken design with three factor and three levels comprising a total of 17 tests. During optimization tests in an experiment with extraction conditions as (time:21/66 min, temperature:49/80 and solution to solid ratio: 32/33) the higest phenolic 105/76 mg Gallic acid (standard of phenolic compound) per g of dry material compound was recorded. Considering the results and graphs temperature was indetified as the most effective factor. In addition, the graphs show that increasing behavior of phenolic compound and extract is achieved when time and incremental. In addition to fulin test, DPPH test was also carried out for investigating antioxidant activity. In DPPH test methanolic extract with IC₅₀= 417/74 µg/ml had the highest antioxidant activity.