Isolation and Identification of Tannin-degrading Bacteria from Native Sheep and Goat Feces in Kohgiloye and Boyer-Ahmad Province

Abstract

Oak trees cover a wide range of forests in Iran and most of them are located in Kohgiloye and Boyer-Ahmad province (K.B). The high tannin is one of the anti-nutritional characteristics of acorn for domestic animal. Wide range of gut microorganisms could tolerate tannin and reduce it by producing tannase enzyme. This study was carried out to isolate and identify the tannin resistance bacteria (tannase enzyme producing bacteria) from feces samples of domestic sheep and goat. For this purpose, twenty six feces samples of native sheep and goats were collected through the province. Firstly, samples were diluted and then 0.1 ml of each suspension (dilutions) was cultivated on the tannin-treated brain heart infusion agar media containing tannin and then was incubated at 37 C for 72 hours under anaerobic conditions. The colonies were isolated and identified by biochemical experiments and API (20 STREP) test. The results showed that five strains of <em>Streptococcus</em> <em>pneumonia</em> and 2 strains of <em>Streptococcus</em> <em>bovis</em> having tannin hydrolysis activity.