Antimicrobial activity of Trachyspermum ammi essential oil against human bacterial

Abstract

The aim of this study was to evaluate the antimicrobial activities of <em>Trachyspermum ammi</em>, essential oil against different kinds of microorganisms by microtiter plate method. All 36 isolates of (12 <em>Klebsiella pneumoniae</em>, 12 <em>E.coli</em> and 12 <em>Staphylococcus aureus</em>) isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections were evaluated. In this study, the essential oil of <em>Trachyspermum ammi</em> obtained by hydrodistillation for 2.5-3 h using a Clevenger-type apparatus and the minimum inhibitory concentrations were determined to characterize the antimicrobial activities of this essential oil. The results showed <em>E. coli </em>isolates were resistance to 4 of the antibiotics including ceftazidime (50%) cefixime (41.6%), tetracyclin(75%), erythromycin(58.3%). However <em>k. </em><em>pneumonia</em> isolates were resistance to 3 of the agent including ceftazidime(33.3%) ,cefixime(58.3%), erythromycin(75%) and <em>S.aureus </em>isolates <em> </em>were resistance to 6 of the agent including cefixime (33.3%), trimethoprim-sulfamethoxazol(41.66%), penicillin(50%), oxacillin(3.3%), ceftazidime(66.6%) and vancomycin(8.3%) and the MIC value was also determined against all the tested bacteria. The highest MIC values of essential oil were determined 100ppm against <em>E. coli </em>and highest MIC value for <em>K.pneumoniae </em>was 250ppm. In conclusion, it seems that <em>Trachyspermum</em><em> ammi</em> essential oil could inhibit the growth of all of the tested bacteria.