Volume 8, Issue 4
مرور بر اساس
ارسال های اخیر
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Inhibition of ackA and pta genes using two specific antisense RNAs reduced acetate accumulation in batch fermentation of E. coli BL21 (DE3)
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Expression of foreign proteins in E. coli is normally inhibited by exogenous production of acetate. To overcomethis problem, various strategies have been proposed and tested to reduce the extent of acetate accumulation.
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Serratia marcescens B4A chitinase product optimization using Taguchi approach
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Chitinase production by newly isolated Serratia marcescens B4A was optimized following Taguchi'sarray methods. Twenty-three bacterial isolates were screened from shrimp culture ponds in the South ofIran. A ...
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Bacterial overexpression of the human interleukin-2 in insoluble form via the pET Trx fusion system
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Selection of a system for successful recombinant protein production is important. The aim of this study wasto produce high levels of human interleukin-2 (hIL-2) in soluble form. To this end, the pET32a vector in
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Callus induction and shoot regeneration from epicotyl explants of ethnomedicinally important Caesalpinia bonduc (L.) Roxb
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS medium
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Functional screening of phosphatase-encoding genes from bacterial sources
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Phosphatase (APase) enzymes including phytases have broad applications in diagnostic kits, poultryfeeds, biofertilizers and plant nutrition. Because of high levels of sequence diversity among phosphatases,an ...
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Comparison of proliferation and osteoblast differentiation of marrow-derived mesenchymal stem cells on nano- and micro-hydroxyapatite contained composite scaffolds
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Bones constructed by tissue engineering are being considered as valuable materials to be used for regeneration of large defects in natural bone. In an attempt to prepare a new bone construct, in this study, proliferation ...
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Glucose 6-phosphate dehydrogenase deficiency in Tehran, Zanjan and Sistan-Balouchestan provinces:prevalence and frequency of Mediterranean variant of G6PD
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an enzymopathy affecting about 400 millionpeople worldwide. The distribution of G6PD deficiency and the molecular genetics of this enzyme vary widelyamong ...
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Dopamine-synthesizing neurons: An overview of their development and application for cell therapy
(National Institute of Genetic Engineering and Biotechnology, 2010-10-01)Cell-gene therapy is a dynamic constituent of novel medical biotechnology. Neurodegenerative disordersin which damage to or demise of specific brain cell types plays central role, are clear examples of disease
