Behenic Acid Monolayer and Bilayer Assemblies- A Study of Concanavalin A (Con A) Adsorption and its Interaction with Dextran Using Surface Plasmon Resonance Spectroscopy and Microscopy

Abstract

<em>Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as well as the interaction between deposited Con A with dextran molecules in the presence of Ca²⁺ and Mn²⁺ ions was investigated. The results obtained, show that no significant interaction occurs between the adsorbed Con A and dextran in the case of B.A. bilayer, while a significant binding takes place in the monolayer.</em>