Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase

Abstract

<em>In this study, decolorization of </em><em>the </em><em>diazo dye, Reactive Black 5</em><em> (RB5</em>) <em>in a </em><em>Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism</em><em> was</em><em> assumed for the </em><em>enzymatic decolorization</em><em>. </em><em>E</em><em>xperiment</em><em>s</em><em>were conducted </em><em>at different RB5 and hydrogen peroxide</em><em>concentrations in a batch manner</em><em>to estimate the intrinsic kinetic parameters. </em><em>These parameters were used for the modeling of decolorization in a </em><em>continuous reactor</em><em> that was compared with </em><em>experimental </em><em>results. An acceptable agreement was observed between the model and experimental data.</em>