MCR of the quenching of the EEM of fluorescence of Aflatoxins (B1, G1) by Gold nanoparticles

Abstract

In This research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. The quenching of the fluorescence of excitation emission matrices (EEM) of two type of aflatoxins (B1, G1), provoked by the gold nanoparticles, was studied by principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS). These aflatoxins show quenching in the presence of the gold-labeled antibodies. Solutions containing a constant concentration of aflatoxins and variable concentrations of gold-labeled antibodies have been prepared and the fluorescence spectra have been recorded by scanning excitation and emission wavelengths. Excitation and emission spectra and quenching profiles were extracted from EEM using MCR-ALS. Applying suitable constraints lead to satisfactory results for the determination of aflatoxins in all samples.