Preparation of antibacterial electrospun Poly lactic-co–glycolic acid nanofibers containing Hypericum Perforatum with bedsore healing property and evaluation of its drug release performance

Abstract

Skin drug delivery systems with controlled release are suitable means for the local transfer of pharmaceutical compounds to the damaged and healthy layers of skin. Nanofibrous membrane prepares uniform moisture in the wound environment with less accumulation of fluid secretion due to its variable pore size. Electrospinning takes advantage of using herbal extracts in the form of electrospun nanofibrous mats as an alternative for skin grafts. In this study, after preparing optimized fibrous mats of Poly lactic-co – glycolic acid (PLGA), characterization and identification of their chemical structure was carried out by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). The drug release of the optimized electrospun nanofibrous mats and the kinetics of drug release were investigated in vitro. Then, wound dressing performance, antibacterial property, cell adhesion and biocompatibility of nanofibrous scaffolds prepared by Methyl Thiazolyl Tetrazolium (MTT) assays as well as important features of suitable wound dressing including porosity, Water Vapor Transmission Rate, and swelling percentage of nanofibrous mats were evaluated in vitro. Nanofibrous mats containing <em>Hypericum perforatum </em>extract without any bead with burst drug release kinetics followed Higuchi kinetic model with proper regression coefficient (PLGA-E₁₀= 0.8, PLGA-E₃₀= 0.76). The results of the antibacterial activity of nanofibrous mats against Gram positive bacteria (<em>S. aureus</em>) as well as the results of the cell culture test and in vitro biodegradability tests on these mats showed good potential of composite scaffolds as antimicrobial coverage for wounds with Gram positive bacteria infectious agent.