Biogenic Approach using Sheep Milk for the Synthesis of Platinum Nanoparticles: The Role of Milk Protein in Platinum Reduction and Stabilization

Abstract

<span class="fontstyle0">Bio-directed synthesis of nanoparticles is an interesting field of rapid advancement for biologists,<br />chemists and materials scientists, especially in light of efforts to find out green methods of inorganic<br />material synthesis. In the present study, green synthesis of platinum nanoparticles (PtNPs) using sheep<br />milk is reported for the first time. By adjusting the concentrations of chloroplatinic acid (H</span><span class="fontstyle0" style="font-size: 7pt;">2</span><span class="fontstyle0">PtCl</span><span class="fontstyle0" style="font-size: 7pt;">6</span><span class="fontstyle0">) and<br />milk in aqueous solutions, spherical PtNPs were obtained at room temperature. The nanoparticles<br />obtained were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), high-resolution<br />Transmission Electron Microscopy (TEM) and X-Ray diffraction (XRD). The spherical particles obtained<br />have an average size 9.0 nm as shown by XRD pattern and TEM analysis. Fourier Transform Infra-Red<br />(FTIR) measurements were carried out to identify the possible biomolecules responsible for capping and<br />efficient stabilization of the PtNPs synthesized by milk. The carboxylic acid group libration and amide I<br />and II bands revealed the binding of protein with platinum surface through the amine group rather than<br />the carboxyl group</span><span class="fontstyle0" style="color: #ff0000;">. </span><span class="fontstyle0">Among a wide variety of biological resources which are introduced for reducing Pt<br />ions into PtNPs, green approaches like using sheep milk have outstanding benefits for synthesize<br />biological metal nanoparticles due to their eco-friendly phenomenon and very less amounts of<br />cytotoxicity. PtNPs green synthesized in this study are potential candidates to use in drug discovery and<br />gene transfer technologies.</span>