Fabrication and evaluation of gelatin nanoparticles for delivering of anti - cancer drug

Abstract

The aim of present study was to prepare gelatin nanoparticle for drug and gene delivery applications. These nanoparticles were prepared by two-step desolvation method. The body distribution of colloidal drug delivery systems was mainly influenced by two physicochemical properties namely particle size and surface characteristics. The influence of several factors on the fabrication process including the amount of desolvating agent, concentration of gelatin, temperature, the amount of glutaraldehyde, organic solvent addition rate and agitation speed was investigated herein. The smallest size of achieved nanoparticles was 168 nm and the largest size was 460 nm which is suitable for drug delivery. The amount of cross linker and organic solvent addition rate have less effect on produced nanoparticle size. The study indicated that a minimum size could be obtained with temperature 60º C, 75 ml acetone and 250 μL glutaraldehyde. To characterize the nanoparticle sample, AFM and SEM were employed whilst size distribution was measured by photon correlation spectroscopy. The mechanistic of the optimum conditions for preparing protein nanoparticles as well as their characterization are discussed.