Characterization of antioxidant and surface active properties of gelatin hydrolysates obtained from croaker fish skin

Abstract

<span>In this study, antioxidant and surface-active properties of gelatin hydrolysates (GH) were evaluated. Gelatin obtained from croaker fish (</span><em>Johnius dussumieri</em><span>) skin was hydrolysed by the application of visceral proteases extracted from gastrointestinal (GI) tract of two freshwater fish species rohu (</span><em>Labeo rohita</em><span>)and catla (</span><em>Catla catla</em><span>). The resultant GH was designated as ‘gelatin hydrolysate-rohu protease' (GH-RP) and ‘gelatin hydrolysate-catla protease' (GH-CP). Surface-active properties (foaming and emulsion) of GH-RP were significantly higher than GH-CP at concentrations studied (P < 0.05). The GH obtained by enzymatic hydrolysis treatment was fractionated using Ultrafiltration and a total number of five fractions were collected unfractionated (UNF), >10 kDa, 5-10 kDa, 3-5 kDa, 1-3 kDa and <span>50</span><span> value of DPPH radical scavenging activity of fraction 1-3 kDa from GH-RP and fraction </span></span>