Persian Sage (<i>>Salvia Rhytidia</i) Essential Oil can Ameliorate the Renal Ischemia-Reperfusion Injuries in Rat

Abstract

Objective- In the present study the role of essential oil of <em>Salvia rhtidia</em> (SR), in ameliorating renal ischemia reperfusion (IR) injuries is evaluated.<br /> Design- Experimental in vivo study.<br /> Animals- 48 male healthy Wistar rats between 250-300 grams.<br /> Procedures- The animals were randomly divided into eight groups of six rats. In ischemic groups (groups A-D), under general anesthesia both renal pedicles were approach from ventral midline and occluded for 40 min by Rumel tourniquet. Groups A and B received SR essential oil 48, 24 and 1 h before operation, whereas group C and D received the same volume of normal saline (NS). In Sham groups (E-H) all the procedures were like groups A-D, except occluding the renal pedicles. The period of reperfusion in groups A, C, E, and G was 1hour, whereas in groups B, D, F, and H was 24 hours, when blood samples were taken to evaluate blood urea nitrogen (BUN) and creatinine followed by sacrificing the animals for histopathological and ultrastructural studies.<br /> Results- IR resulted in increase in BUN and Creatinine significantly. The most increase was in group D (40min ischemia, 24h reperfusion with NS). Histopathogically and ultramicroscopically the most prominent changes including severe acute tubular necrosis (ATN), hemorrhage and thiroidization, cytoplasmic vacuolization, swollen mitochondria with reduction in the number of cristae, pyknotic nuclei with abnormal chromatin condensation were also in group D.<br /> Conclusion and Clinical Relevance- This study showed that SR essential oil can protect the kindly from IR injuries, due to antioxidative effects of the drug.