Toxicity Properties of Silver Nanoparticles on Lactate Dehydrogenase Activity and Histological Changes of Heart and Embryo Tissues in Pregnant Mice (NMRI)

Abstract

The rapid advancement of nanotechnology enables us to use nanoscale particles. This material in terms of physical, chemical and biological characteristics are unique compared to larger particles. Increased cell division, apoptosis, oxidative stress has been associated with toxic effects of nano-silver. The aim of this study was to evaluate histipotological changes and enzyme activity in nanoparticle silver trated pregnant NMRI mice. These experimental were performed on 35 NMRI mice used for treatment with Ag Nps. The average weight of the animals was 30 ± 3 g that divided into five groups of seven were injected intraperitoneally. After mentioned treatment, the blood sampling was done of NMRI. The collected tissues were washed with saline and fixed in Boin΄s fluid and stained with hematoxylin and eosin for histopathology evaluation. After data collection, statistical analysis was done using SAS software. Histological observations showed that the silver nanoparticles had a major effect on fetal development in each experimental groups compared to the control group. No change of histological characteristics of heart tissues was observed in Ag-nps groups as compared to the control group. Different concentrations of silver nanoparticles increased levels of enzyme lactate dehydrogenase , but no significant differences were observed between control and treated groups (P<0.05). Toxicity of silver nanoparticles injected intraperitoneally into the experimental groups were evaluated which had unfavorable effects on embryonic development. So, further investigates are suggested to predict AgNPs toxicity.