Evaluation of Physicochemical Methods for Dormancy Breakage and Germination of Datura stramonium Seeds

Abstract

<em>Datura stramonium</em> L. broadly performed in plant virology study has a significant seed germination difficulty due to its seed dormancy. This study was evaluated to find a simple seedling germination method intended for <em>D. stramonium</em> seeds as a traditional healthy detector plant. A factorial experiment organized as completely randomized design (CRD) in triplicate, determining results regarding seedling scarification, gibberellins (GA₃) along with potassium nitrate (KNO₃) at different concentrations from 0 to 2000 ppm. The particular germination rate along with radicle and plumule length scarified seed had been significantly affected using concentrations of GA₃ as well as KNO₃. After five days of treatment, radicle and plumule length and the percentage of germination were recorded, uncovering the real key function involving mechanical seed scarification method as being a requirement for germination of <em>D. stramonium</em>. The highest germination rate occurred at a concentration of 100 ppm GA₃ and 500 ppm for KNO₃ (63%).